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Module 6

- Microbial inoculants are preparations containing beneficial microorganisms that improve soil fertility and crop productivity.
- Azospirillum is an associative nitrogen fixer found in the rhizosphere of cereals like maize, wheat, and sorghum.
- Phosphate Solubilizing Microorganisms (PSM) convert insoluble phosphate compounds into soluble forms available to plants.
- Pseudomonas fluorescens acts as a bio-control agent against many soil-borne plant pathogens.
- Trichoderma spp. are fungi used as biofungicides for seed and soil treatment.
- Blue-green algae (BGA) fix atmospheric nitrogen in rice fields under submerged conditions.
- Cyanobacteria are oxygenic photosynthetic prokaryotes containing chlorophyll a and phycobiliproteins.
- Frankia forms actinorhizal symbiosis with non-leguminous plants like Alnus, Casuarina, and Myrica.
- Rhizobium-legume symbiosis is an example of mutualism where both partners benefit.
- Nod factors are lipochitooligosaccharides produced by Rhizobium that induce nodule formation.
- Nitrogenase enzyme catalyzes the reduction of N₂ to NH₃ under anaerobic conditions.
- Leghemoglobin in nodules regulates oxygen concentration for nitrogenase activity.
- Ammonification is the microbial conversion of organic nitrogen into ammonia.
- Nitrification is the oxidation of ammonia to nitrate via nitrite by Nitrosomonas and Nitrobacter.
- Denitrification converts nitrate into nitrogen gas, leading to nitrogen loss from soil.
- Sulphur oxidizing bacteria like Thiobacillus convert sulfides to sulfates.
- Iron bacteria like Gallionella and Leptothrix oxidize ferrous iron to ferric form.
- Manganese bacteria oxidize Mn²⁺ to Mn⁴⁺ and are important in soil weathering.
- Silicate bacteria release soluble silica from insoluble silicate minerals, improving soil fertility.
- Biofertilizers are substances containing living microorganisms that promote plant growth by increasing the supply or availability of nutrients.
- The main types of biofertilizers include Rhizobium, Azospirillum, Azotobacter, Blue-Green Algae, and Phosphate Solubilizing Microorganisms (PSM).
- Rhizobium inoculant is crop-specific and mainly used for leguminous crops.
- Azotobacter is a free-living nitrogen-fixing bacterium found in the rhizosphere of non-leguminous plants.
- Azospirillum forms loose associations with roots of cereals and grasses.
- Blue-Green Algae (BGA) biofertilizer is widely used in rice fields.
- Azolla, a water fern, has a symbiotic relationship with Anabaena azollae, a nitrogen-fixing cyanobacterium.
- Phosphate solubilizing bacteria (PSB) include Bacillus megaterium and Pseudomonas striata.
- Mycorrhizae are symbiotic associations between fungi and roots that enhance nutrient uptake.
- Ectomycorrhiza forms an external sheath around roots (e.g., Pinus).
- Endomycorrhiza (Vesicular Arbuscular Mycorrhiza – VAM) penetrates root cells to form arbuscules and vesicles.
- VAM fungi belong mainly to the genera Glomus, Acaulospora, and Gigaspora.
- Biofertilizers improve soil fertility, reduce chemical fertilizer use, and promote sustainable agriculture.
- Composting is the microbial decomposition of organic matter under controlled conditions.
- Aerobic composting produces humus-like material useful as manure.
- Vermicomposting uses earthworms (like Eisenia foetida) to enhance decomposition.
- Biogas production involves anaerobic digestion of organic waste by methanogenic bacteria.
- The main gases in biogas are methane (60–70%) and carbon dioxide (30–40%).
- Methanobacterium and Methanococcus are important methane-producing microorganisms.
- Silage is preserved green fodder prepared through anaerobic fermentation by lactic acid bacteria.
- Lactic acid bacteria (LAB) such as Lactobacillus plantarum are used in silage fermentation.
- Microbial pesticides (biopesticides) use microorganisms to control insect pests and diseases.
- Bacillus thuringiensis (Bt) produces Cry toxins that kill specific insect larvae.
- Beauveria bassiana is an entomopathogenic fungus effective against many insect pests.
- Trichoderma harzianum acts as a biofungicide and plant growth promoter.
- Nuclear Polyhedrosis Virus (NPV) controls Helicoverpa armigera and other lepidopteran pests.
- Biocontrol agents are eco-friendly alternatives to chemical pesticides.
- Antagonism is the mechanism by which one microorganism suppresses another (e.g., Trichoderma vs Fusarium).
- Antibiotics are secondary metabolites produced by microbes that inhibit other microorganisms.
- Streptomyces species are known producers of antibiotics like Streptomycin and Tetracycline.
- Bioleaching is the use of microorganisms to extract metals from ores.
- Thiobacillus ferrooxidans is used in copper bioleaching.
- Microbial biotechnology uses microbes in industrial production of enzymes, organic acids, and alcohols.
- Yeast (Saccharomyces cerevisiae) is used in fermentation industries to produce alcohol and bread.
- Lactic acid bacteria produce curd, yogurt, and cheese by fermenting milk.
- Acetobacter aceti converts ethanol to acetic acid (vinegar).
- Penicillium chrysogenum produces the antibiotic Penicillin.
- Aspergillus niger is used for the commercial production of citric acid.
- Clostridium acetobutylicum produces acetone and butanol.
- Microbial enzymes like amylase, protease, and cellulase are used in food and textile industries.
- Bioethanol is produced from sugarcane molasses and corn by yeast fermentation.
- Bioremediation uses microorganisms to degrade pollutants and clean up the environment.
- Oil-degrading bacteria like Pseudomonas putida are used for petroleum spill cleanup.
- Phytoremediation involves plants and their associated microbes to detoxify contaminated soils.
- Bioaugmentation refers to the addition of specific microbial strains to enhance biodegradation.
- Biosensors are analytical devices combining biological components with a physicochemical detector.
- Genetically Modified Microorganisms (GMMs) are engineered for enhanced metabolic activities.
- Agrobacterium tumefaciens is used in genetic engineering to transfer genes into plants.
- Ti plasmid of Agrobacterium acts as a natural vector for gene transfer.
- Microbial genetics helps in understanding mutation, recombination, and resistance development in microbes.
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