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Horticulture
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UPCATET PG / M. Sc. Agriculture

Bacteria

  • Definition: Bacteria are unicellular, microscopic, prokaryotic organisms that reproduce mainly by binary fission and lack a true nucleus and membrane-bound organelles.
  • They can be free-living or pathogenic (causing diseases in plants, animals, and humans).

General Characteristics of Plant Pathogenic Bacteria

Character

Description

Nature

Prokaryotic, unicellular

Size

0.5–5 µm

Cell Wall

Present, made of peptidoglycan

Nucleus

Absent (nucleoid present)

Reproduction

Asexual – binary fission

Movement

Many are motile (flagella)

Nutrition

Heterotrophic

Habitat

Soil, water, plant surfaces

Shape

Cocci (spherical), Bacilli (rod), Spirilla (spiral)

 

Shape and Arrangement of Bacteria

Shape

Example

Arrangement

Coccus (spherical)

Streptococcus

Singly or chains

Bacillus (rod)

Bacillus subtilis

Single rods

Spirillum (spiral)

Spirillum volutans

Spiral or helical

Comma-shaped

Vibrio cholerae

Curved rods

 

Flagellar Arrangements (Motility Types)

Type

Description

Example

Monotrichous

Single flagellum

Xanthomonas

Lophotrichous

Tuft of flagella at one end

Pseudomonas

Amphitrichous

One or more flagella at both ends

Spirillum

Peritrichous

Flagella all over

Erwinia

Atrichous

No flagella (non-motile)

Agrobacterium

 

Reproduction

  • By Binary Fission (asexual).
  • No sexual reproduction like fungi, but genetic exchange occurs via:
    • Conjugation
    • Transformation
    • Transduction

 

Detection of Bacteria

  • Ooze Test: Milky or yellow ooze from infected tissue (bacterial streaming).
  • Gram Staining:
    • Gram +ve: Retains violet color (thick peptidoglycan)
      Clavibacter michiganensis
    • Gram –ve: Red/pink (thin peptidoglycan)
      Xanthomonas, Pseudomonas, Erwinia

 

Important Plant Diseases Caused by Bacteria

Disease

Causal Organism

Host

Citrus canker

Xanthomonas citri

Citrus

Black rot

Xanthomonas campestris pv. campestris

Crucifers

Bacterial blight

Xanthomonas oryzae pv. oryzae

Rice

Fire blight

Erwinia amylovora

Apple, pear

Soft rot

Erwinia carotovora

Potato, vegetables

Leaf spot

Pseudomonas syringae

Beans

Crown gall

Agrobacterium tumefaciens

Many dicots

Moko disease

Ralstonia (Pseudomonas) solanacearum

Banana

Angular leaf spot

Xanthomonas campestris pv. malvacearum

Cotton

 

Important Facts & MCQs

Question

Answer

Father of Bacteriology

Louis Pasteur

First bacterial plant disease discovered

Fire blight of pear (Erwinia amylovora)

Gram staining developed by

Christian Gram (1884)

Bacteria reproduce by

Binary fission

Ooze test used for detecting

Bacteria

Cell wall composition

Peptidoglycan

Storage material

Glycogen

DNA arrangement

Circular, naked (no histones)

Sensitive to Tetracycline

Mycoplasma / Phytoplasma

Shape of Xanthomonas

Rod-shaped

Causes gall formation

Agrobacterium tumefaciens

Disease suppressed by chlorine

Downy mildew of Bajra

Mycoplasma vs Bacteria

Mycoplasma → No cell wall; Bacteria → Have cell wall

 

MYCOPLASMA / PHYTOPLASMA 

Feature

Mycoplasma / Phytoplasma

Nature

Cell wall-less bacteria

Sensitive to

Tetracycline

Shape

Pleomorphic (variable)

Habitat

Phloem tissue

Transmission

Leafhoppers / planthoppers

Examples

Little leaf of brinjal, Phyllody of sesame, Grassy shoot of sugarcane

 

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