Course Content
Rural Sociology and Educational Psychology 2 (2+0)
B. Sc. Agriculture (Hons.) Ist. Semester (Six Deam Commitee of ICAR)
Importance of Bio-regulators in Horticultural Crops

Introduction

  • The growth and development of horticultural crops are controlled by hormones, also called plant growth substances or bio-regulators.
    These naturally occurring or synthetic compounds influence cell division, elongation, flowering, fruiting, ripening, and dormancy.
  • Their judicious use in horticultural crops improves growth, yield, quality, and marketability.

 

Definition

  • Bio-regulators (or Plant Growth Regulators – PGRs) are organic compounds, other than nutrients, which in small quantities influence the physiological processes of plants such as growth, differentiation, and development.
  • They can be:
  • Naturally produced (plant hormones)
  • Synthetic chemicals that mimic or inhibit natural hormones.

 

Classification of Bio-regulators

Group

Examples

General Function

1. Auxins

IAA, IBA, NAA, 2,4-D

Cell elongation, rooting, fruit set, prevent fruit drop

2. Gibberellins

GA₃, GA₄

Stem elongation, breaking dormancy, fruit size improvement

3. Cytokinins

Kinetin, Benzyl Adenine (BA), Zeatin

Cell division, delay senescence, improve fruit quality

4. Ethylene

Ethephon (Ethrel), Ethylene gas

Fruit ripening, flower induction, abscission

5. Abscisic Acid (ABA)

Abscisic acid

Induces dormancy, stress resistance

6. Growth Retardants / Inhibitors

CCC (Cycocel), Alar, Paclobutrazol

Control vegetative growth, promote flowering

7. Growth Promoters (Combinations)

Mixtures of auxin + cytokinin + GA₃

Used in tissue culture and fruit growth regulation

 

 

Major Functions of Bio-regulators in Horticultural Crops

Vegetative Growth Regulation

  • Auxins promote cell elongation and root initiation: e.g., IBA (2000 ppm) for rooting of guava, grapes, and pomegranate cuttings.
  • Gibberellins (GA₃) stimulate stem elongation and leaf expansion.
  • Cytokinins promote cell division and shoot proliferation.
  • Growth retardants (CCC, paclobutrazol) reduce excessive vegetative growth (e.g., in mango, citrus).

 

Flower Induction and Regulation

  • Gibberellins (GA₃) induce flowering in non-flowering plants (e.g., pineapple).
  • Paclobutrazol (PP333) promotes flowering by reducing vegetative growth in mango and citrus.
  • Ethephon (Ethrel) induces flowering in pineapple (50–100 ppm).
  • Cytokinins help in bud break and synchronization of flowering.

 

Fruit Set and Fruit Development

  • Auxins (NAA, 2,4-D) induce parthenocarpic (seedless) fruit set in tomato, citrus, and grapes.
  • GA₃ (25–50 ppm) increases fruit set and berry size in grapes.
  • NAA (20–40 ppm) improves fruit retention and reduces premature fruit drop in mango and citrus.

 

Fruit Thinning and Sizing

  • NAA (10–20 ppm) and 2,4-D (10 ppm) used for fruit thinning in apple and citrus to improve size and quality.
  • GA₃ (50–100 ppm) increases fruit length and weight in grape, apple, and citrus.

 

Fruit Ripening and Degreening: Ethrel (100–500 ppm) releases ethylene, which promotes: Uniform ripening in banana, mango, and pineapple. Degreening in citrus fruits for better market appearance.

 

Delay in Ripening (Postharvest Management)

  • Cytokinins (BA, kinetin) delay senescence and maintain green color of leaves and fruits.
  • GA₃ delays ripening in banana and tomato, extending shelf life.

 

Breaking Dormancy

  • Gibberellins (GA₃, 100 ppm) break bud dormancy in grapes, peach, and potato tubers.
  • Thiourea and KNO₃ are also used as dormancy-breaking agents in temperate fruits.

 

Prevention of Premature Fruit Drop

  • NAA (20–40 ppm) or 2,4-D (10 ppm) applied before harvesting prevent fruit drop in mango, citrus, and apple.

 

Rooting of Cuttings and Grafting Success

  • IBA (2000 ppm) and NAA (500–1000 ppm) promote rooting in hardwood and semi-hardwood cuttings.
  • Improves survival rate in propagation of guava, grapes, pomegranate, and rose.

 

Parthenocarpy Induction

  • Auxins (NAA), Gibberellins (GA₃), and 2,4-D induce seedless fruit formation.
  • Example: GA₃ induces parthenocarpy in grapes and cucumber; NAA in citrus.

 

Color Development and Quality Improvement

  • Ethrel enhances fruit color (degreening).
  • Cytokinins and GA₃ improve size, sweetness, and shelf life of fruits.
  • Calcium sprays with PGRs improve firmness and reduce cracking.

 

Postharvest Life Extension

  • Cytokinins delay leaf yellowing and fruit aging.
  • GA₃ maintains firmness and delays softening.
  • Ethrel regulates uniform ripening for market preparation.

 

Role of Bio-regulators in Different Horticultural Crops

Crop

Bio-regulator & Concentration

Purpose / Effect

Mango

Paclobutrazol (5–10 ml/tree)

Induces flowering, controls alternate bearing

Grape

GA₃ (25–50 ppm)

Increases berry size and fruit set

Citrus

2,4-D (10 ppm), NAA (20 ppm)

Prevents fruit drop

Banana

Ethrel (100 ppm)

Uniform ripening

Papaya

GA₃ (100 ppm)

Increases fruit size

Apple

NAA (10 ppm)

Fruit thinning

Pineapple

Ethrel (50–100 ppm)

Flower induction

Tomato

2,4-D (10 ppm)

Induces parthenocarpy

Guava

NAA (100 ppm)

Reduces fruit drop

Chrysanthemum / Ornamental

CCC (500 ppm)

Controls plant height, enhances flowering

 

Methods of Application of Bio-regulators

Method

Description / Example

Foliar spray

Common method for NAA, GA₃, and ethrel applications.

Soil drenching

Used for paclobutrazol (mango, citrus).

Seed or cutting treatment

Auxins like IBA for rooting.

Paste application / smearing

For localized bud treatment.

Injection / trunk absorption

For slow, controlled uptake (in perennials).

 

Advantages of Using Bio-regulators

Benefit

Explanation

1. Early and uniform flowering

Important for synchronization and yield stability.

2. Reduced fruit drop

Enhances yield.

3. Improved fruit size and color

Better marketability.

4. Seedless fruit production

Induces parthenocarpy.

5. Stress tolerance

Helps plants withstand drought or heat stress.

6. Controlled growth

Maintains desired plant shape and size.

7. Enhanced rooting and propagation

Improves vegetative multiplication.

 

 

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