Bio-fertilizers: classification and characteristics
Introduction
- Modern sustainable agriculture focuses on improving productivity while maintaining soil health and minimizing environmental pollution.
- Bio-fertilizers play a vital role in this by enhancing nutrient availability to crops through biological processes.
Definition
- Bio-fertilizers are living microorganisms which, when applied to seed, plant surface, or soil, promote plant growth by increasing the availability or supply of primary nutrients to the host plant.
- According to the National Project on Organic Farming (NPOF, India):
- “Bio-fertilizers are products containing carrier-based (solid or liquid) living microorganisms which are agriculturally useful in terms of nitrogen fixation, phosphorus solubilization, or nutrient mobilization.”
Importance of Bio-fertilizers
- Enhance soil fertility through biological nitrogen fixation, phosphorus solubilization, and mobilization of nutrients.
- Reduce dependence on chemical fertilizers.
- Improve soil microbial activity and soil structure.
- Promote eco-friendly and sustainable farming.
- Cost-effective and safe for the environment.
- Increase nutrient uptake and crop yield naturally.
Mechanism of Bio-fertilizers
Bio-fertilizers act through various mechanisms:
- Biological Nitrogen Fixation (BNF) – Conversion of atmospheric N₂ into plant-usable form (NH₃).
- Phosphorus Solubilization – Conversion of insoluble P (like rock phosphate) into soluble forms.
- Phosphorus Mobilization – Mycorrhizal fungi help in mobilizing P from deeper soil layers.
- Production of Growth Hormones – Indole acetic acid (IAA), gibberellins, cytokinins.
- Decomposition of Organic Matter – Enhances nutrient release.
- Disease Suppression – By producing antibiotics or competing with pathogens.
Classification of Bio-fertilizers
- Bio-fertilizers are classified based on the type of nutrient they fix or mobilize, and the microorganisms involved.
- Nitrogen-Fixing Bio-fertilizers
|
Type |
Microorganism |
Symbiotic/Free-living |
Host/Crop |
|
Rhizobium |
Rhizobium spp. |
Symbiotic |
Leguminous crops (pulses, clovers, beans, peas) |
|
Azospirillum |
Azospirillum brasilense, A. lipoferum |
Associative |
Cereals (maize, wheat, sorghum, millets) |
|
Azotobacter |
Azotobacter chroococcum, A. vinelandii |
Free-living |
Non-legumes (cotton, maize, vegetables) |
|
Blue-Green Algae (BGA) |
Anabaena, Nostoc, Aulosira |
Free-living / Symbiotic |
Wetland rice fields |
|
Azolla-Anabaena |
Azolla pinnata + Anabaena azollae |
Symbiotic (fern + cyanobacteria) |
Rice fields |
|
Frankia |
Frankia spp. |
Symbiotic |
Non-legume trees (Alnus, Casuarina) |
- Phosphorus-Solubilizing and Mobilizing Bio-fertilizers
|
Type |
Microorganism |
Function |
Host/Crop |
|
Phosphate Solubilizing Bacteria (PSB) |
Bacillus megaterium, Pseudomonas striata |
Solubilize insoluble phosphates (Ca, Fe, Al phosphates) |
All crops |
|
Phosphate Solubilizing Fungi (PSF) |
Aspergillus awamori, Penicillium spp. |
Produce organic acids to dissolve P |
All crops |
|
Mycorrhiza (VAM – Vesicular Arbuscular Mycorrhiza) |
Glomus, Gigaspora, Acaulospora |
Mobilize P, Zn, Cu, and water from deeper layers |
Cereals, fruits, trees |
- Bio-fertilizers for Other Nutrients
|
Nutrient |
Microorganism |
Function |
|
Sulphur-oxidizing bacteria |
Thiobacillus thiooxidans |
Converts elemental S → sulphate (SO₄²⁻) |
|
Zinc-solubilizing bacteria |
Bacillus subtilis, Pseudomonas fluorescens |
Solubilize Zn compounds |
|
Potassium-mobilizing bacteria (KMB) |
Frateuria aurantia |
Mobilize K from mica, feldspar |
Plant Growth-Promoting Rhizobacteria (PGPR)
- Include beneficial bacteria that colonize the rhizosphere and promote plant growth through:
- Nutrient solubilization.
- Hormone production (IAA, GA, Cytokinins).
- Antagonism against pathogens.
- Stress tolerance.
Examples: Pseudomonas fluorescens, Bacillus subtilis, Serratia marcescens.
- Organic Waste Decomposers
|
Type |
Microorganism |
Function |
|
Cellulose Decomposers |
Trichoderma, Aspergillus, Chaetomium |
Break down crop residues into compost |
|
Bio-composting cultures |
Trichoderma viride, Pleurotus, Phanerochaete |
Speed up decomposition and nutrient recycling |
- Characteristics of an Efficient Bio-fertilizer
An ideal bio-fertilizer should possess the following qualities:
- High microbial population (at least 10⁷–10⁹ viable cells/g or ml).
- Strain specificity to particular crops or soil conditions.
- High survival rate under storage and field conditions.
- Non-toxic and eco-friendly to plants, animals, and humans.
- Quick establishment and multiplication in the rhizosphere.
- Compatibility with fertilizers and pesticides.
- Efficient nutrient fixation or solubilization ability.
- Longer shelf life (preferably 6–12 months).
- Carrier material should maintain microbial viability (e.g., peat, lignite, charcoal).
- Easy application (seed treatment, soil application, or root dipping).
- Forms of Bio-fertilizers
|
Form |
Description |
Advantages |
|
Solid-based (carrier-based) |
Peat, lignite, compost, charcoal |
Easy to apply, low cost |
|
Liquid-based |
Nutrient solution with stabilizers and protectants |
Higher shelf life (up to 2 years), better microbial count |
|
Granular formulations |
Used for soil application |
Uniform distribution and slow release |
- Methods of Application
|
Method |
Description |
Used For |
|
Seed treatment |
Seeds coated with bio-fertilizer slurry before sowing |
Rhizobium, Azospirillum, PSB |
|
Seedling root dip |
Roots dipped in bio-fertilizer solution before transplanting |
Paddy, vegetables |
|
Soil application |
Mixed with compost and applied to soil |
Azotobacter, PSB, VAM |
|
Foliar spray |
Sprayed on foliage for quick response |
PGPR, Liquid formulations |
- Advantages of Bio-fertilizers
- Improve soil fertility and structure.
- Reduce chemical fertilizer requirement by 25–50%.
- Increase yield by 10–30%.
- Maintain soil microbial balance.
- Reduce greenhouse gas emissions.
- Enhance soil organic carbon and water-holding capacity.
- Support sustainable and organic farming systems.
- Limitations
- Require proper storage and handling.
- Sensitive to high temperature and sunlight.
- Effectiveness depends on soil type, pH, and moisture.
- Slower in response compared to chemical fertilizers.
- Specific to crops and ecological conditions.
- Examples of Commercial Bio-fertilizer Products in India
|
Product Name |
Type |
Company/Agency |
|
Bio-NPK |
Mixed culture (N fixers + P solubilizers + K mobilizers) |
ICAR-NBAIM, Mau |
|
Rhizobium Culture |
N-fixer |
Various State Agricultural Universities |
|
Phospho-rich Biofertilizer (PRB) |
PSB + compost |
NCOF, Ghaziabad |
|
Azospirillum Biofertilizer |
N-fixer |
TNAU, Coimbatore |
|
Azolla |
N-fixer |
Wetland rice fields (manual cultivation) |
