Course Content
Rural Sociology and Educational Psychology 2 (2+0)
B. Sc. Agriculture (Hons.) Ist. Semester (Six Deam Commitee of ICAR)

Bio-fertilizers: classification and characteristics

 

Introduction

  • Modern sustainable agriculture focuses on improving productivity while maintaining soil health and minimizing environmental pollution.
  • Bio-fertilizers play a vital role in this by enhancing nutrient availability to crops through biological processes.

 

Definition

  • Bio-fertilizers are living microorganisms which, when applied to seed, plant surface, or soil, promote plant growth by increasing the availability or supply of primary nutrients to the host plant.
  • According to the National Project on Organic Farming (NPOF, India):
  • “Bio-fertilizers are products containing carrier-based (solid or liquid) living microorganisms which are agriculturally useful in terms of nitrogen fixation, phosphorus solubilization, or nutrient mobilization.”

 

Importance of Bio-fertilizers

  • Enhance soil fertility through biological nitrogen fixation, phosphorus solubilization, and mobilization of nutrients.
  • Reduce dependence on chemical fertilizers.
  • Improve soil microbial activity and soil structure.
  • Promote eco-friendly and sustainable farming.
  • Cost-effective and safe for the environment.
  • Increase nutrient uptake and crop yield naturally.

 

Mechanism of Bio-fertilizers

Bio-fertilizers act through various mechanisms:

  • Biological Nitrogen Fixation (BNF) – Conversion of atmospheric N₂ into plant-usable form (NH₃).
  • Phosphorus Solubilization – Conversion of insoluble P (like rock phosphate) into soluble forms.
  • Phosphorus Mobilization – Mycorrhizal fungi help in mobilizing P from deeper soil layers.
  • Production of Growth Hormones – Indole acetic acid (IAA), gibberellins, cytokinins.
  • Decomposition of Organic Matter – Enhances nutrient release.
  • Disease Suppression – By producing antibiotics or competing with pathogens.

 

Classification of Bio-fertilizers

  • Bio-fertilizers are classified based on the type of nutrient they fix or mobilize, and the microorganisms involved.
  • Nitrogen-Fixing Bio-fertilizers

Type

Microorganism

Symbiotic/Free-living

Host/Crop

Rhizobium

Rhizobium spp.

Symbiotic

Leguminous crops (pulses, clovers, beans, peas)

Azospirillum

Azospirillum brasilense, A. lipoferum

Associative

Cereals (maize, wheat, sorghum, millets)

Azotobacter

Azotobacter chroococcum, A. vinelandii

Free-living

Non-legumes (cotton, maize, vegetables)

Blue-Green Algae (BGA)

Anabaena, Nostoc, Aulosira

Free-living / Symbiotic

Wetland rice fields

Azolla-Anabaena

Azolla pinnata + Anabaena azollae

Symbiotic (fern + cyanobacteria)

Rice fields

Frankia

Frankia spp.

Symbiotic

Non-legume trees (Alnus, Casuarina)

 

  • Phosphorus-Solubilizing and Mobilizing Bio-fertilizers

Type

Microorganism

Function

Host/Crop

Phosphate Solubilizing Bacteria (PSB)

Bacillus megaterium, Pseudomonas striata

Solubilize insoluble phosphates (Ca, Fe, Al phosphates)

All crops

Phosphate Solubilizing Fungi (PSF)

Aspergillus awamori, Penicillium spp.

Produce organic acids to dissolve P

All crops

Mycorrhiza (VAM – Vesicular Arbuscular Mycorrhiza)

Glomus, Gigaspora, Acaulospora

Mobilize P, Zn, Cu, and water from deeper layers

Cereals, fruits, trees

 

  • Bio-fertilizers for Other Nutrients

Nutrient

Microorganism

Function

Sulphur-oxidizing bacteria

Thiobacillus thiooxidans

Converts elemental S → sulphate (SO₄²⁻)

Zinc-solubilizing bacteria

Bacillus subtilis, Pseudomonas fluorescens

Solubilize Zn compounds

Potassium-mobilizing bacteria (KMB)

Frateuria aurantia

Mobilize K from mica, feldspar

 

Plant Growth-Promoting Rhizobacteria (PGPR)

  • Include beneficial bacteria that colonize the rhizosphere and promote plant growth through:
    • Nutrient solubilization.
    • Hormone production (IAA, GA, Cytokinins).
    • Antagonism against pathogens.
    • Stress tolerance.

Examples: Pseudomonas fluorescens, Bacillus subtilis, Serratia marcescens.

 

  • Organic Waste Decomposers

Type

Microorganism

Function

Cellulose Decomposers

Trichoderma, Aspergillus, Chaetomium

Break down crop residues into compost

Bio-composting cultures

Trichoderma viride, Pleurotus, Phanerochaete

Speed up decomposition and nutrient recycling

 

  1. Characteristics of an Efficient Bio-fertilizer

An ideal bio-fertilizer should possess the following qualities:

  • High microbial population (at least 10⁷–10⁹ viable cells/g or ml).
  • Strain specificity to particular crops or soil conditions.
  • High survival rate under storage and field conditions.
  • Non-toxic and eco-friendly to plants, animals, and humans.
  • Quick establishment and multiplication in the rhizosphere.
  • Compatibility with fertilizers and pesticides.
  • Efficient nutrient fixation or solubilization ability.
  • Longer shelf life (preferably 6–12 months).
  • Carrier material should maintain microbial viability (e.g., peat, lignite, charcoal).
  • Easy application (seed treatment, soil application, or root dipping).

 

  1. Forms of Bio-fertilizers

Form

Description

Advantages

Solid-based (carrier-based)

Peat, lignite, compost, charcoal

Easy to apply, low cost

Liquid-based

Nutrient solution with stabilizers and protectants

Higher shelf life (up to 2 years), better microbial count

Granular formulations

Used for soil application

Uniform distribution and slow release

 

  1. Methods of Application

Method

Description

Used For

Seed treatment

Seeds coated with bio-fertilizer slurry before sowing

Rhizobium, Azospirillum, PSB

Seedling root dip

Roots dipped in bio-fertilizer solution before transplanting

Paddy, vegetables

Soil application

Mixed with compost and applied to soil

Azotobacter, PSB, VAM

Foliar spray

Sprayed on foliage for quick response

PGPR, Liquid formulations

 

  1. Advantages of Bio-fertilizers
  • Improve soil fertility and structure.
  • Reduce chemical fertilizer requirement by 25–50%.
  • Increase yield by 10–30%.
  • Maintain soil microbial balance.
  • Reduce greenhouse gas emissions.
  • Enhance soil organic carbon and water-holding capacity.
  • Support sustainable and organic farming systems.

 

  1. Limitations
  • Require proper storage and handling.
  • Sensitive to high temperature and sunlight.
  • Effectiveness depends on soil type, pH, and moisture.
  • Slower in response compared to chemical fertilizers.
  • Specific to crops and ecological conditions.

 

  1. Examples of Commercial Bio-fertilizer Products in India

Product Name

Type

Company/Agency

Bio-NPK

Mixed culture (N fixers + P solubilizers + K mobilizers)

ICAR-NBAIM, Mau

Rhizobium Culture

N-fixer

Various State Agricultural Universities

Phospho-rich Biofertilizer (PRB)

PSB + compost

NCOF, Ghaziabad

Azospirillum Biofertilizer

N-fixer

TNAU, Coimbatore

Azolla

N-fixer

Wetland rice fields (manual cultivation)

 

 

 

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