Course Content
Fundamentals of Plant Pathology
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Fundamentals of Agricultural Extension Education
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B.Sc. Ag. II Semester
    About Lesson
    1. Phytoplasmas

    Definition

    Phytoplasmas are wall-less prokaryotic pathogens restricted to the phloem tissue of plants and transmitted by phloem-feeding insects like leafhoppers, psyllids, and planthoppers. They are associated with several plant diseases, particularly those involving stunted growth, yellowing, and witches’ broom symptoms.

    Characteristics

    • Morphology: Pleomorphic (variable shape) due to the absence of a rigid cell wall.
    • Size: Small (0.2–0.8 µm in diameter).
    • Genome: Highly reduced, around 530–1350 kb, making them dependent on their hosts.
    • Habitat: Found in the phloem tissue of infected plants and the bodies of insect vectors.
    • Transmission:
      • Spread by phloem-feeding insects like leafhoppers and psyllids.
      • Can also be transmitted through grafting, infected cuttings, or vegetative propagation.

     

    • Symptoms:
      • Yellowing (chlorosis)
      • Witches’ broom (proliferation of shoots)
      • Stunting
      • Floral abnormalities (e.g., phyllody—conversion of flowers into leaf-like structures)

     

    Examples of Diseases Caused by Phytoplasmas

    1. Grassy Shoot Disease of Sugarcane: Caused by Candidatus Phytoplasma sacchari.
    2. Little Leaf of Brinjal: Caused by Candidatus Phytoplasma aurantifolia.
    3. Coconut Lethal Yellowing: Caused by Candidatus Phytoplasma palmae.
    4. Aster Yellows: Caused by Candidatus Phytoplasma asteris.
    5. Sesame Phyllody: Caused by Candidatus Phytoplasma sesami.

     

     

    1. Spiroplasmas

    Definition

    Spiroplasmas are wall-less, helical-shaped prokaryotes found in phloem tissue of plants and the hemolymph (blood) of their insect vectors. They are distinct due to their spiral or helical morphology and motility.

    Characteristics

    • Morphology: Helical or spiral-shaped and motile.
    • Size: Small (0.2–0.3 µm wide and up to 5 µm long).
    • Genome: Larger than phytoplasmas, approximately 1 Mb.
    • Habitat: Found in the phloem of plants and hemolymph of insect vectors.
    • Transmission:
      • Transmitted by leafhoppers and planthoppers.
      • Can be experimentally transmitted through mechanical inoculation.

     

    • Symptoms:
      • Stunting
      • Yellowing (chlorosis)
      • Reduced vigor and sometimes wilting

     

    Examples of Diseases Caused by Spiroplasmas

    1. Citrus Stubborn Disease: Caused by Spiroplasma citri.
    2. Corn Stunt Disease: Caused by Spiroplasma kunkelii.
    3. Periwinkle Disease: Caused by Spiroplasma phoeniceum.

     

    Comparison Between Phytoplasmas and Spiroplasmas

    Feature

    Phytoplasmas

    Spiroplasmas

    Morphology

    Pleomorphic

    Helical or spiral-shaped

    Size

    0.2–0.8 µm

    0.2–5 µm

    Cell Wall

    Absent

    Absent

    Genome Size

    Smaller (530–1350 kb)

    Larger (~1 Mb)

    Habitat

    Phloem of plants

    Phloem of plants and hemolymph of insects

    Transmission

    Phloem-feeding insects (e.g., leafhoppers, psyllids)

    Phloem-feeding insects (e.g., leafhoppers)

    Examples of Diseases

    Little leaf of brinjal, Grassy shoot of sugarcane

    Citrus stubborn, Corn stunt

     

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