Photosynthesis
- Photosynthesis is the process of converting light energy into chemical energy.
- The overall equation is:
6CO₂ + 12H₂O → C₆H₁₂O₆ + 6O₂ + 6H₂O. - Site of photosynthesis – Chloroplast.
- Light reaction occurs in grana; Dark reaction (Calvin cycle) occurs in stroma.
- Hill reaction demonstrates oxygen evolution during photosynthesis.
- Photophosphorylation is the formation of ATP using light energy.
- C₃ plants fix CO₂ via Rubisco enzyme (Calvin cycle).
- C₄ plants fix CO₂ via PEP carboxylase enzyme.
- First stable product of C₃ pathway – 3-phosphoglyceric acid (3-PGA).
- First stable product of C₄ pathway – Oxaloacetic acid (OAA).
- CAM plants open stomata at night to fix CO₂ (e.g., Pineapple, Opuntia).
- Optimum temperature for photosynthesis in C₃ plants: 25°C; C₄ plants: 35°C.
- Photosynthetic quotient (PQ) = O₂ evolved / CO₂ absorbed.
- Photorespiration occurs only in C₃ plants.
- Photorespiration site: Chloroplast → Peroxisome → Mitochondria.
Respiration
- Respiration is oxidation of food to release energy (ATP).
- Site of respiration –
- Glycolysis occurs in
- Krebs cycle occurs in mitochondrial matrix.
- End product of aerobic respiration – CO₂ and H₂O.
- End product of anaerobic respiration – Alcohol / Lactic acid.
- RQ (Respiratory Quotient) = CO₂ evolved / O₂ consumed.
- RQ of carbohydrate = 1, fat = 7, protein = 0.8.
- RQ of organic acids >
- Pasteur effect – inhibition of glycolysis by
Transpiration
- Transpiration – loss of water in vapor form through stomata.
- Types: Stomatal, Cuticular, Lenticular.
- Transpiration mainly occurs through
- Transpiration ratio = Water transpired / Dry matter produced.
- Antitranspirants reduce transpiration (e.g., PMA, Kaolin, CCC).
- Potometer is used to measure rate of transpiration.
- Stomata open in light and close in darkness.
- Guard cells contain chloroplasts; surrounding epidermal cells do not.
- Opening of stomata is due to turgor pressure in guard cells.
- Hydathodes are involved in
Water Relations
- Water potential (Ψw) – potential energy of water per unit volume.
- Pure water has Ψw =
- Osmosis – movement of water from low solute to high solute concentration.
- Plasmolysis – shrinkage of cytoplasm due to water loss.
- Imbibition – absorption of water by colloids (seeds, cell walls).
- Turgor pressure – pressure exerted by cell sap on the cell wall.
- Wilting point – when plants cannot absorb water from soil.
- Permanent Wilting Point (PWP) – water unavailable to plants.
- Field Capacity – maximum water soil can hold after drainage.
- Water uptake mainly through root hairs.
Growth and Development
- Growth – irreversible increase in size or mass.
- Development – progression from seed to maturity.
- Cell elongation occurs in the meristematic region.
- Auxins promote cell elongation and apical dominance.
- Gibberellins (GA₃) promote stem elongation and seed germination.
- Cytokinins promote cell division and delay senescence.
- Abscisic acid (ABA) induces dormancy and stress tolerance.
- Ethylene promotes fruit ripening and leaf abscission.
- Phototropism – growth towards light.
- Geotropism – growth in response to gravity.
- Apical dominance – suppression of lateral buds by apical bud (Auxin effect).
- Bolting – elongation of internodes in rosette plants due to GA₃.
- Vernalization – induction of flowering by low temperature.
- Photoperiodism – response of plants to day length.
- Short-day plants flower when day length < critical period (e.g., Rice).
- Long-day plants flower when day length > critical period (e.g., Wheat).
- Neutral plants – flowering not affected by photoperiod (e.g., Cotton).
- Phytochrome is the photoreceptor pigment controlling photoperiodism.
Plant Stress Physiology
- Drought stress causes closure of stomata and reduced photosynthesis.
- Heat stress leads to protein denaturation and enzyme inactivation.
- Cold stress reduces membrane fluidity and chlorophyll content.
- Salt stress causes ion toxicity (Na⁺, Cl⁻) and osmotic imbalance.
- Waterlogging causes anaerobic respiration in roots.
- ABA increases during drought and stress.
- Proline accumulation – an indicator of stress tolerance.